Screening Libraries

Compound Collections

The choice of which chemical libraries to screen should be guided by the goals of the project and the biological system of interest.

Biochemical screens seeking novel chemical entities capable of binding to a protein and modulating its function would be best served by diverse collections of drug-like small molecules such as the Maybridge HitFinder or the Chembridge MicroFormats. Confirmation and optimization of hits and can be managed by enlisting advice, collaborations, or through further contract work with the HTSC.

Cellular assays can reveal insights into the biological regulation of your phenotype with “known actives” and “approved drugs” collections. Screening characterized collections serves as a set of baseline controls to help hone the assay system and avoid false positives resulting from undesired biological effects. For example, one may wish to develop counter-screens to weed out cytotoxic compounds from consideration in a neuroscience screen. Many of the compounds in these collections have been validated in animals, allowing a progression to disease models by removing the hurdle of toxicology, pharmacokinetic, and pharmacodynamic studies.

If investigators would like to develop novel chemical compounds as hits with a long term view toward drug discovery, it is advisable to screen large and diverse collections of drug-like small molecules.

Compound Libraries

Library    Source Size Compound Type
1 Apoptosis    SelleckChem 95 Known
2 Autophagy    SelleckChem 154 Known
3 Bioactive Lipids    Enzo 190 Known
4 Epigenetics    SelleckChem 151 Known
5 GPCR    SelleckChem 256 Known
6 Ion Channel Ligand    SelleckChem 56 Known
7 Stem Cell Signaling    SelleckChem 81 Known
8 Kinase Inhibitors    SelleckChem 436 Known
9 ICCB Bioactives    Enzo 480 Known
10 Pharmakon    Microsource 1,760 Known Drugs
11 Spectrum Collection    Microsource 2,560 Known Drugs/ Bioactives/Nat Product
12 NCI Diversity Set    NCI 1,584 Diverse (request directly from NCI)
13 Cysteine-Focused    Life Chemicals 3,200 Cysteine-Focused Covalent Inhibitors
14 Maybridge Hit-Finder    Thermo 14,400 Diverse
15 Chembridge Microformats    Chembridge 100,000 Diverse

 

HTSC